Fig. 3
From: Genome sequencing and secondary metabolism of the postharvest pathogen Penicillium griseofulvum
Patulin production and relative expression of key patulin genes in P. griseofulvum PG3. a Patulin production in vitro. b Patulin production in apple. c. Relative gene expression (RGE) of patK, patG, patL and patN in PG3. The gene expression is relative to the geometric mean of the four P. griseofulvum reference genes: the ribosomal 28S RNA, the 37S ribosomal protein (PGRI_092740), the beta-tubulin (PGRI_052690) and the histone H3 (PGRI_044770). Spore suspensions of strains were inoculated on the centre of 55 mm PDA plates and incubated at 24 °C in the dark for up to 10 days post inoculation (dpi). Error bars indicate standard deviations of three biological replicates. Different letters above the columns indicate a significant difference determined by Tukey's Multiple Comparison Test (p < 0.05)