Fig. 5

Subcellular localization of A. millepora Bcl-2 family proteins. a, b Plasmid constructs encoding A. millepora Bcl-2 family proteins fused with EGFP and plasmids encoding mCherry as a marker to visualize organelles, such as mitochondria, endoplasmic reticulum (ER) and Golgi apparatus, were transiently co-transfected. In the expression of pro-apoptotic AmBax and AmBokA, the FLAG/XlBclXL protein was co-expressed to prevent apoptosis of the transfected cells. After 2 days of cultivation, cells were washed and fixed. Both green and red fluorescent images of transfected cells were acquired using confocal microscopy and also merged into one image, resulting in an orange color in areas of co-expression. Scale bars represent 10 μm