Fig. 3

ChIP-qPCR validation of selected TrmBL1 binding sites identified by ChIP-Seq. ChIP enrichment is presented as % input. The mean with SD of at least three replicates of IP is shown for all analysed genomic loci. a TrmBL1 ChIP of cells grown under gluconeogenic condition (pyruvate 1). The genes PF1882 (aaa + atpase) and PF1602 (gdh) represent the negative controls for the anti-TrmBL1 IgG. b TrmBL1 ChIP of cells grown under glycolytic growth condition (starch 1). The genes PF1882 (aaa + atpase) and PF1602 (gdh) represent the negative controls for the anti-TrmBL1 IgG. c ChIP with a Phr specific antibody using cells grown on pyruvate (gluconeogenic conditions). The gene PF1882 (aaa + atpase; [29]) is the positive control for the anti-Phr IgG, whereas the genes PF1784 (pfk) and PF1602 (gdh) are the negative controls for the anti-Phr IgG. d ChIP with a Phr specific antibody using cells grown on starch (glycolytic conditions). The gene PF1882 (aaa + atpase) is the positive control for the anti-Phr IgG, whereas the genes PF1784 (pfk) and PF1602 (gdh) are the negative controls for the anti-Phr IgG