Fig. 7
From: Genome-wide binding analysis of the transcriptional regulator TrmBL1 in Pyrococcus furiosus
Deletion of a 16Â kb fragment encoding the TM system and TrmB. a southern blot analysis using BamHI and SmaI digested P. furiosus genomic DNA. DNA was isolated from cells after recultivation and adaption to glycolytic (starch) or gluconeogenic (pyruvate) growth conditions. The result of two independent recultivations is shown. The probe, specific for PF1753, detects a 3Â kb fragment, while the PF1743 specific probe binds to a 7Â kb fragment. b copy number analysis of the genes PF1743 and PF1753 in P. furiosus cells grown under the following conditions: glycolytic (starch and maltose) or gluconeogenic (pyruvate). The gene PF1784 was used as calibrator and the recultivation culture as reference. The mean of three biological replicates including SD is shown