Fig. 2
From: Interaction network of tobacco etch potyvirus NIa protein with the host proteome during infection
Infectivity of TEV recombinant clones with tagged NIa. a and b N. benthamiana plants non-inoculated (mock) and agroinoculated with wild-type TEV (TEV-wt) and TEV clones with TST-NIa at five different positions (TEV-TSTNIa1 to TEV-TSTNIa5). Pictures of representative plants were taken at 9 (a) or 26 (b) days postinoculation (dpi). c RT-PCR analysis of the viral progenies from plants infected with TEV-wt (lanes 2 to 4, 9 to 11 and 16 to 18), TEV-TSTNIa1 (lanes 5 to 7), TEV-TSTNIa2 (lanes 12 to 14) and TEV-TSTNIa5 (lanes 19 to 21). The control RT-PCR reaction (lane 1) contained all primers and no template. Lanes 8, 15 and 22, DNA marker ladder with sizes on the right side in kbp. Red arrows point the PCR products delayed as a consequence of insertion of the TST cDNA in the viral progenies