Transcriptome analysis reveals differentially expressed genes associated with germ cell and gonad development in the Southern bluefin tuna (Thunnus maccoyii)

Table 2 Gonad cell samples, treatment and RNA extraction quality and quantity details

Sample^a	Tissue	Preparation treatment	RIN^b	RNA conc. (ng/μL)
SBT_T2	Testis	Crude	8.10	100.00
SBT_T3	Testis	Crude	8.00	100.00
SBT_T4	Testis	Crude	8.50	100.00
SBT_T7	Testis	Crude	7.20	100.00
SBT_T11	Testis	Crude	7.30	100.00
SBT_TP2	Testis	Enriched	9.10	40.00
SBT_TP3	Testis	Enriched	9.60	100.00
SBT_TP4	Testis	Enriched	8.90	100.00
SBT_TP5	Testis	Enriched	9.30	100.00
SBT_TP7	Testis	Enriched	8.70	100.00
SBT_O6	Ovary	Crude	6.50	100.00
SBT_O8	Ovary	Crude	7.10	100.00
SBT_O9	Ovary	Crude	6.50	100.00
SBT_O10	Ovary	Crude	7.80	100.00
SBT_O12	Ovary	Crude	7.60	100.00
SBT_OC6	Ovary	Enriched	NA	100.00
SBT_OC8	Ovary	Enriched	NA	100.00
SBT_OC9	Ovary	Enriched	NA	100.00
SBT_OC10	Ovary	Enriched	NA	100.00
SBT_OC12	Ovary	Enriched	8	85.00

^aSample name explanation: letters after the underscore stand for the tissue the cells derived from: ‘T’ – Testis; ‘TP’ – Testis cells enriched with Percoll; ‘O’ – Ovary; ‘OC’ – Ovary filtered Cells. The number at the end of each sample id correlates to fish id in Table 1. ^bRNA integrity number (RIN), as determined by 2100 BioAnalyzer

ISSN: 1471-2164