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Table 2 Gonad cell samples, treatment and RNA extraction quality and quantity details

From: Transcriptome analysis reveals differentially expressed genes associated with germ cell and gonad development in the Southern bluefin tuna (Thunnus maccoyii)

Samplea Tissue Preparation treatment RINb RNA conc. (ng/μL)
SBT_T2 Testis Crude 8.10 100.00
SBT_T3 Testis Crude 8.00 100.00
SBT_T4 Testis Crude 8.50 100.00
SBT_T7 Testis Crude 7.20 100.00
SBT_T11 Testis Crude 7.30 100.00
SBT_TP2 Testis Enriched 9.10 40.00
SBT_TP3 Testis Enriched 9.60 100.00
SBT_TP4 Testis Enriched 8.90 100.00
SBT_TP5 Testis Enriched 9.30 100.00
SBT_TP7 Testis Enriched 8.70 100.00
SBT_O6 Ovary Crude 6.50 100.00
SBT_O8 Ovary Crude 7.10 100.00
SBT_O9 Ovary Crude 6.50 100.00
SBT_O10 Ovary Crude 7.80 100.00
SBT_O12 Ovary Crude 7.60 100.00
SBT_OC6 Ovary Enriched NA 100.00
SBT_OC8 Ovary Enriched NA 100.00
SBT_OC9 Ovary Enriched NA 100.00
SBT_OC10 Ovary Enriched NA 100.00
SBT_OC12 Ovary Enriched 8 85.00
  1. aSample name explanation: letters after the underscore stand for the tissue the cells derived from: ‘T’ – Testis; ‘TP’ – Testis cells enriched with Percoll; ‘O’ – Ovary; ‘OC’ – Ovary filtered Cells. The number at the end of each sample id correlates to fish id in Table 1. bRNA integrity number (RIN), as determined by 2100 BioAnalyzer