Fig. 2

Construction of the Tager 104 Genome. a The Tager 104 genome was constructed using indicated methods, with each step incorporating new genomic data (Inputs). First, Tager 104 paired-end reads from Illumina MiSeq sequencing were submitted to CLC Bio (Contigging). Resulting contigs were scaffolded using reads from PacBio RS sequencing (Scaffolding). Due to the inability to further close scaffolds, PacBio RS data alone was instead contigged using HGAP assembly (Contigging 2). The eight remaining contigs were then closed using Lucigen NxSeq mate-pair libraries with the SSPACE algorithm. Single-headed arrows represent events where resulting data from the previous step was submitted to algorithms in the subsequent step (Method). Steps in which results were interchangeable are indicated by two-headed arrows. The assembly is described in more detail in “Methods.” b In order to confirm the completed genome, Lucigen NxSeq libraries were submitted with separate 2 × 250 paired-end reads in an independent SPAdes construction devoid of previous read data from panel A. Results were identical to the finished genome