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Fig. 2 | BMC Genomics

Fig. 2

From: Rapid identification of causative insertions underlying Medicago truncatula Tnt1 mutants defective in symbiotic nitrogen fixation from a forward genetic screen by whole genome sequencing

Fig. 2

Phenotypic characterization of NF11217 and NF10547 plants. Plants were grown on aeroponic chambers in the presence of 5 mM ammonium nitrate for 5 days, followed by 5 days without nitrogen, and subsequently inoculated with S. meliloti Rm41 hemA:lacZ. Phenotyping was performed 15 days post inoculation. a Whole plants. WT leaves and petioles are green while the mutants’ leaves and petioles are reddish purple, caused by anthocyanin accumulation during nitrogen deficiency. Scale bar = 1 cm. b Visible nodule phenotype. WT nodules are pink; in comparison the mutants’ nodules are brownish or white. Scale bar = 0.5 mm. c Rhizobial occupancy nodule phenotype. The nodules were fixed and stained with X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galacto-pyranoside), sectioned to 100-micron-thickness, and imaged using bright field. Scale bar = 100 μm

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BMC Genomics

ISSN: 1471-2164

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