Fig. 4

RT-qPCR experiments of a selected set of lncRNAs in male adults. G1: high expression with chromatin signatures (11 lncRNAs); G2: low expression with chromatin signatures (11 lncRNAs); G3: high expression without chromatin signatures (10 lncRNAs); and G4: low expression without chromatin signatures (10 lncRNAs). Three negative controls (un-transcribed region 1, 2, and 3) were all around zero. Stars were used to highlight the lncRNAs that were not from the databases (Orange stars: the selected lncRNAs from Young et al. [18]. Blue stars: the lncRNAs from the present study). The horizontal line indicated the cutoff (−delta Ct ≥2) used to define a validated lncRNA. Green stars: the transcripts that are now annotated as other types of transcripts by FlyBase, and thus were removed from the list of the curated lncRNAs in the present study