Fig. 4

Nur77 deficiency results in decreased MMP activity and enhanced collagen content. a The top 25 canonical pathways associated with differentially expressed genes comparing Nur77-KO with WT BMMs were identified by IPA. The line graph represents the ratio of differentially expressed genes from the dataset to the genes present in each canonical pathway. The pathways are ranked from highest to lowest degree of association between genes from the data set with the pathways by the p-value, calculated by a right tailed Fisher Exact Test. The bar graph represents the -log(p-value) and the threshold represents p = 0.05. b The -log10 p-values of the MMPs (blue), TIMPs (red) and collagens (green) were plotted against their corresponding log2 fold changes (Nur77-KO vs WT) in unstimulated (left panel) and LPS stimulated (right panel) BMM. c MMP activity in BMM lysates derived from WT and Nur77-KO mice was determined by zymography and MMP9 activity was quantified (right panel). c, control; LPS, 24 h stimulation with 100 ng/ml LPS. d Collagen content measured in WT and Nur77-KO BMM stimulated for 24 h with 100 ng/ml LPS. *p < 0.05, **p < 0.01, ***p < 0.001