Fig. 2
From: High-throughput discovery of post-transcriptional cis-regulatory elements
Isolating cells undergoing differential gene regulation. a dsRed intensity was measured in cells expressing the reporter construct shown in Fig. 1. The cells with the middle 50 % of dsRed intensity (shown in red and gray) were used for subsequent steps; GFP+ cells with the middle 25 % of dsRed intensity (shown in gray) were also collected as a stringent background set. b Five GFP-positive sub-populations were sorted from the red/gray population in A: 0–10 % (green; lowest GFP bin), 20–30 % (yellow), 40–60 % (gray), 70–80 % (blue), and 90–100 % (purple; highest GFP bin). For each, 5x105 cells were collected, in duplicate. Additionally, 106 GFP+ cells were sorted and retained (GFP-ALL). c Sorted cells had heritable differences in GFP expression. Three weeks after sorting, the populations’ fluorescence were measured via FACS. The GFP intensities for the indicated GFP-sub populations (color-coded to match panel B) are shown overlaying the GFP intensities for the GFP-ALL population; the number of cells were normalized to the mode GFP intensity