Run identity | Initial number of reads generated | Initial number of amplicons pooled [number of replicates] | Reads post filtering on Galaxy [% of initial reads] | Final number of amplicons over read threshold of 120 [number of replicates] | Final mean [and range] of read coverage per amplicon |
---|---|---|---|---|---|
454 Titanium lane 1 | 138,185 | 80 [24] | 104,937 [75.9 %] | 52 [16] | 1,448.5 [120–23,492] |
454 Titanium lane 2 | 98,474 | 102 [21] | 78,642 [79.9 %] | 30 [0] | 1,841.9 [120–11,048] |
454 Titanium lane 3 | 90,355 | 102 [30] | 38,903 [43.1 %] | 101 [29] | 299.8 [165–471] |
454 Titanium lane 4 | 103,559 | 115 [19] | 29,769 [28.8 %] | 81 [13] | 210.7 [120–638] |
454 Titanium lane 5 | 108,867 | 95 [19] | 43,921 [40.3 %] | 79 [12] | 393.8 [120–1,313] |
Ion Torrent run 1 | 607,318 | 120 [12] | 277,195 [45.6 %] | 99 [5] | 1,885.6 [120–7,190] |
Ion Torrent run 2 | 573,126 | 120 [16] | 198,604 [34.6 %] | 99 [8] | 1,415.2 [120–5,727] |
Ion Torrent run 3 | 693,065 | 121 [14] | 341,675 [49.3 %] | 101 [5] | 2,534.6 [120–15,851] |
All Runs | 2,176,290 | 855 [155] | 930,067 [42.7 %] | 642 [88] | 1087.8 [120–23,492] |