Fig. 1
From: Characterization of an extensive rainbow trout miRNA transcriptome by next generation sequencing
Identification and annotation pipeline of rainbow trout miRNAs using sRNA-sequencing. Fastq files were first cleaned from low quality and adapter sequence. The redundancy was removed from the 38 sample files for the annotation process (Panel 1). Unique sequences were then blasted against rRNA, tRNA and RNA database to remove already known RNA sequences that are not miRNA (Panel 2). A Blast against miRBase database predicts the already identified miRNAs in other species (Panel 2). In parallel, reads were mapped onto the rainbow trout genome to eliminate any contaminant sequences and characterize the miRNA loci. Novel pre-miRNA were also characterized with the specific miRNAs features (Panel 3)