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Fig. 5 | BMC Genomics

Fig. 5

From: Identification and functional analyses of sex determination genes in the sexually dimorphic stag beetle Cyclommatus metallifer

Fig. 5

Gene annotation of isotig 04317. a Predicted gene model of isotig 04317 (Cmtra), and expressed transcripts in females and males. The coding sequences are in gray, stop codons are indicated by black arrowheads. The open arrowhead indicates the start codon. Arrows indicate forward and reverse primer locations for RT-PCR (b) Expression patterns of Cmtra in females and males. Template cDNAs were derived from Stage 3 prepupae. H: head, L: leg, W: wing. GAPDH was used as a positive control. (c) Alignment of conserved 25 amino acid sequences of the putative auto regulation domain [37] of the Tra protein. d NJ tree of Tra protein from the conserved 25 amino acid auto-regulation domain

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BMC Genomics

ISSN: 1471-2164

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