Fig. 1
From: Circulating miRNA signatures of early pregnancy in cattle
Optimisation of methodology for profiling miRNAs in bovine plasma. a RT-qPCR data plots (with mean ± SEM) showing abundance of the exogenous miRNA, cel-miR-39-3p, in plasma samples after RNA extraction using 3 different kits as indicated. b RNA yield from 1.05 mL bovine plasma samples using TRIzol LS. c Results of RT-qPCR quantification of miR-451 in plasma (mean ± SEM) using different RNA volumes for reverse-transcription; highest reaction efficiency was obtained using 2 μL of RNA in a 10 μL cDNA synthesis reaction