Fig. 3From: RNA sequencing to characterize transcriptional changes of sexual maturation and mating in the female oriental fruit fly Bactrocera dorsalis qRT-PCR confirmation of the differentially expressed genes between mature virgin females and the immature individuals. Total RNAs from B. dorsalis at three different physiological stages were isolated, including the immature (newly emergent within 24 h), virgin (14-day-old before mating) and mated (14-day-old post-mating). Relative transcript levels are calculated by real-time PCR using the 16 s rRNA gene as the standard control. Different letters represent significant differences (p < 0.05, Duncan’s test) among samples. Three independent biological replicates were performed for each sampleBack to article page