A novel method of transcriptome interpretation reveals a quantitative suppressive effect on tomato immune signaling by two domains in a single pathogen effector protein

Table 1 Treatments used for RNA-Seq analysis of PTI suppression by physical domains of AvrPto

Treatment	Concentration	Comment	Average total reads (in millions)	Average reads mapped (in percent)
Mock	10 mM MgCl₂, 0.02 % silwet	No bacteria	15.2	93.1
D29E pCPP45::avrPto	OD₆₀₀ 0.02	Wild-type	15.6	93.6
D29E pCPP45::avrPto(I96A)	OD₆₀₀ 0.02	Core domain (CD) inactivated	16.3	94.1
D29E pCPP45::avrPto(2xA)^a	OD₆₀₀ 0.02	C-terminal domain (CTD) inactivated	16.6	94.3
D29E pCPP45::avrPto(I96A, 2xA)^a	OD₆₀₀ 0.02	‘CD⁻/CTD⁻‘	14.9	93.8

ISSN: 1471-2164