Fig. 2

NC2 shutoff provokes an increase in non-coding RNA transcripts. Metagene representation of the average transcription profiles for each analyzed gene class. Ten units correspond to 200 bp. In between TSS (transcription start site) and polyA (polyadenylation site), the entire gene lengths are normalized to 20 units (20–40 on the abscissa scale). The ordinates scale represents the ratio between the mutant and wild-type signals. a–b) Canonical ORFs show an increase in both the short sense transcripts in the 5′ and 3′ flanks and in antisense transcripts when blocking the transcription of both NC2 subunits. c–d) Only minor differences are seen between TATA and TATA-like genes when blocking NC2 transcripts. e–h) Cryptic mapped transcripts (SUTs and CUTs) show similar effects to those observed in canonical genes after shutting down NC2 subunits. a, c, e, g: pGAL-YDR1 shutoff; b, d, f, h: pGAL-BUR6 shutoff. i) Interpretation of the observed results. The cryptic transcripts (red arrows) over a scheme for a canonical yeast gene with its typical nucleosomal organization. Putative SRT (Ssu72-restricted transcripts) and RRT (Rco1-restricted transcripts) are marked. See the main text for a discussion