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Table 1 RNA-seq data validation using qRT-PCR. Fold-change values were calculated using the ∆∆Ct method and normalized to the reference gene RNA 16S for 20 differentially expressed genes. HP: gene that codes for a hypothetical protein

From: RNA-seq analysis of the Rhizobium tropici CIAT 899 transcriptome shows similarities in the activation patterns of symbiotic genes in the presence of apigenin and salt

Gene name Locus tag Nucleotide range RNA-seq qRT-PCR
Apigenin Salt Apigenin Salt
araC1 RTCIAT899_CH06050 1238211_1239707 1.81 6.98 1.60 3.49
rpsL RTCIAT899_CH07390 1516428_1516799 −1.61 12.47 3.20 7.11
araC2 RTCIAT899_CH14150 2846615_2847376 −2.56 −8.00 1.97 2.04
y4wE RTCIAT899_PB00575 97717_98829 8.58 12.17 15.35 16.68
y4wF RTCIAT899_PB00570 96476_97504 3.20 7.11 12.05 10.04
nodA2 RTCIAT899_PB01095 192166_192756 10.30 9.81 13.90 17.00
hsnT RTCIAT899_PB01100 192929_194854 7.27 5.94 25.67 12.50
nodF RTCIAT899_PB01105 194950_195231 13.39 13.43 11.90 13.45
nodE RTCIAT899_PB01110 195232_196440 10.37 11.94 16.74 13.78
nodA1 RTCIAT899_PB01300 235667_236257 6.69 12.00 14.18 17.83
nodB RTCIAT899_PB01305 236254_236913 11.4 19.01 8.55 16.30
nodC RTCIAT899_PB01310 236925_238283 6.69 12.00 11.39 15.94
nodS RTCIAT899_PB01315 238201_238917 7.53 12.89 6.03 10.03
nodU RTCIAT899_PB01320 238953_240680 5.39 10.18 15.29 22.86
nodI RTCIAT899_PB01325 240668_241582 6.59 12.48 7.58 11.89
nodJ RTCIAT899_PB01330 241586_242371 4.98 10.15 3.92 15.83
nodH RTCIAT899_PB01340 242956_243705 3.09 7.6 14.80 17.02
HP RTCIAT899_PB01545 281777_282742 4.27 14.37 8.88 18.11
nodM RTCIAT899_PB02710 502220_504046 2.43 5.85 5.69 12.58
HP RTCIAT899_PC04980 1059004_1060056 1.32 −4.22 1.14 −5.38