RNA-seq analysis of the Rhizobium tropici CIAT 899 transcriptome shows similarities in the activation patterns of symbiotic genes in the presence of apigenin and salt

Table 1 RNA-seq data validation using qRT-PCR. Fold-change values were calculated using the ∆∆Ct method and normalized to the reference gene RNA 16S for 20 differentially expressed genes. HP: gene that codes for a hypothetical protein

Gene name	Locus tag	Nucleotide range	RNA-seq		qRT-PCR
Gene name	Locus tag	Nucleotide range	Apigenin	Salt	Apigenin	Salt
araC1	RTCIAT899_CH06050	1238211_1239707	1.81	6.98	1.60	3.49
rpsL	RTCIAT899_CH07390	1516428_1516799	−1.61	12.47	3.20	7.11
araC2	RTCIAT899_CH14150	2846615_2847376	−2.56	−8.00	1.97	2.04
y4wE	RTCIAT899_PB00575	97717_98829	8.58	12.17	15.35	16.68
y4wF	RTCIAT899_PB00570	96476_97504	3.20	7.11	12.05	10.04
nodA2	RTCIAT899_PB01095	192166_192756	10.30	9.81	13.90	17.00
hsnT	RTCIAT899_PB01100	192929_194854	7.27	5.94	25.67	12.50
nodF	RTCIAT899_PB01105	194950_195231	13.39	13.43	11.90	13.45
nodE	RTCIAT899_PB01110	195232_196440	10.37	11.94	16.74	13.78
nodA1	RTCIAT899_PB01300	235667_236257	6.69	12.00	14.18	17.83
nodB	RTCIAT899_PB01305	236254_236913	11.4	19.01	8.55	16.30
nodC	RTCIAT899_PB01310	236925_238283	6.69	12.00	11.39	15.94
nodS	RTCIAT899_PB01315	238201_238917	7.53	12.89	6.03	10.03
nodU	RTCIAT899_PB01320	238953_240680	5.39	10.18	15.29	22.86
nodI	RTCIAT899_PB01325	240668_241582	6.59	12.48	7.58	11.89
nodJ	RTCIAT899_PB01330	241586_242371	4.98	10.15	3.92	15.83
nodH	RTCIAT899_PB01340	242956_243705	3.09	7.6	14.80	17.02
HP	RTCIAT899_PB01545	281777_282742	4.27	14.37	8.88	18.11
nodM	RTCIAT899_PB02710	502220_504046	2.43	5.85	5.69	12.58
HP	RTCIAT899_PC04980	1059004_1060056	1.32	−4.22	1.14	−5.38

ISSN: 1471-2164