Fig. 8

Relative expression of the eight soybean C4-3 MATE genes in response to Al toxicity. The relative expression of the eight GmMATE genes in the soybean root tips (0–2 mm) in response to Al stress (25 μM AlCl₃) was quantified by qRT-PCR, using soybean GmEF-1α gene as the internal control and 0 μM AlCl₃ (control) as reference. The error bars indicate the standard deviation from three replicates. T: aluminum-tolerant cultivar, KF; S: aluminum-sensitive cultivar, GF. * and ** indicates significant difference in the relative expression level between T and S in response to Al stress for each time point (student’s t-test) at P < 0.05 and P < 0.01, respectively