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Table 3 Differentially methylated 3Kb regions between Holstein and Angus cultures as determined by MIRA-Seq and gene expression data for annotated genes

From: Differential responsiveness of Holstein and Angus dermal fibroblasts to LPS challenge occurs without major differences in the methylome

    

RNA-Seq fold changec

FCa

Chr

Genomic coordinates

Geneb

Hour 0

Hour 2

Hour 8

−2.9

1

8811701–8814700

ADAMTS5

- -

- -

- -

4.2

1

4686501–4689500

LOC100848874

- -

- -

- -

2.0

1

8325701–8328700

LOC526789

- -

- -

- -

−3.4

2

88463334–88466333

- -

- -

- -

- -

−3.4

2

88469334–88472333

- -

- -

- -

- -

2.6

2

18491734–18494733

OSBPL6

- -

1.4

1.5

2.0

5

116626706–116629705

FBLN1

- -

- -

- -

2.3

6

5529182–5532181

- - -

- -

- -

- -

−8.3

7

18532846–18535845

LOC100337044

- -

- -

- -

2.9

8

23178787–23181786

LOC100300143

- -

- -

- -

−2.6

8

34929387–34932386

- -

- -

- -

- -

−2.7

8

78669587–78672586

- -

- -

- -

- -

−2.8

8

12568687–12571686

- -

- -

- -

- -

−4.0

8

79335787–79338786

NTRK2

- -

- -

- -

−2.6

8

98801087–98804086

- -

- -

- -

- -

−2.6

9

20444051–20447050

- -

- -

- -

- -

−2.7

9

93571151–93574150

- -

- -

- -

- -

−4.0

9

88292251–88295250

ULBP1

- -

- -

- -

−2.7

10

32127701–32130700

- -

- -

- -

- -

2.1

12

51081922–51084921

- -

- -

- -

- -

−2.5

13

64236797–64239796

ASIP

−14.0

−15.2

−13.4

2.8

13

48576797–48579796

LRRN4

- -

- -

- -

2.6

14

36500947–36503946

- - -

- -

- -

- -

2.1

15

53581057–53584156

LOC100336675

- -

- -

- -

−2.3

15

9012857–9015856

- -

- -

- -

- -

−7.5

15

27535857–27538856

- -

- -

- -

- -

−2.8

15

55516557–55519556

SERPINH1

- -

- -

- -

−2.4

17

7224294–7227293

MAB21L2

- -

- -

- -

2.2

17

17977294–17980293

MAML3

- -

- -

- -

−2.8

18

60220698–60223697

LOC100848332

- -

- -

- -

−3.6

18

63217498–63220497

LOC783134

- -

- -

- -

3.4

18

33452798–33455797

- -

- -

- -

- -

2.4

19

42556475–42559474

- -

- -

- -

- -

2.3

19

62858675–62861674

AXIN2

- -

- -

- -

−2.4

19

43381475–43384474

CNTNAP1

- -

1.8

2.0

−3.6

19

23655275–23658274

HIC1

- -

1.7

- -

2.2

19

42109475–42112474

KRTAP9-2

- -

- -

- -

2.4

19

62705675–62708674

RGS9

2.0

2.1

2.5

4.8

23

49777993–49780992

- - -

- - -

- - -

- - -

2.8

24

59112931–59115930

CCBE1

−2.3

−2.2

−1.8

2.4

24

62388931–62391930

- - -

- - -

- - -

- - -

2.5

25

27266601–27269600

- - -

- - -

- - -

- - -

2.4

26

23658031–23661030

C26H10orf26

- - -

- - -

1.4

−2.4

26

16776031–16779030

LOC100848660

- - -

- - -

- - -

3.2

27

23720367–23723366

- - -

- - -

- - -

- - -

−2.4

X

63726195–63729194

LOC100848206

2.4

2.6

2.3

2.1

X

111529495–111532494

LOC516666

- - -

- - -

- - -

2.1

X

128195395–128198394

- - -

- - -

- - -

- - -

−2.6

X

55935095–55938094

- - -

- - -

- - -

- - -

  1. aData obtained by MIRA-Seq and presented as fold difference in read count of methylated regions. Positive fold change indicates higher methylation levels in Holstein cultures while negative values are higher methylation in Angus cultures
  2. bDMRs with an associated gene indicate that some portion of the 3Kb region falls within an annotated gene, while - - indicates DMRs that are intergenic. Bolded gene names indicate that the discovered DMR fell within the promoter region of that gene (-2500 to +500bp from gene transcription start site)
  3. cPositive values indicate greater expression in Holstein cultures as compared to Angus