Fig. 1

Pipeline of data from RNA-Seq to DE-lincRNA candidates responding to stripe rust and powdery mildew. Sequence reads were assembled using the Trinity platform and all unigenes were annotated in NCBI, COG, GO and KEGG. Unknown transcripts were filtered using thresholds of ORF length and nucleotide length. After filtering, transcripts were further alignmented with genome sequences downloaded from URGI, and then those genes that have not gap were reserved as putative lincRNA. The differential expression (DE)-lincRNAs were identified on the condition of fold change ≥2 and the false discovery rate (FDR) at 1.0 %. Then, DE-lincRNA were further verified though analysis of corresponding genome sequences by Genscan