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Table 2 Pips identified by Yeast two-hybrid screening

From: Analysis of the interactome of the Ser/Thr Protein Phosphatase type 1 in Plasmodium falciparum

  PlasmoDB Accession numbera Namea Growth on selective plates (number of isolated clones)b RVxF motifsc
In frame genes PF3D7_0107600 serine/threonine protein kinase, putative TDO/A (1)  
PF3D7_0220000 liver stage antigen 3 (LSA3) QDO/A (2) KKVRF
PF3D7_0418300 conserved Plasmodium protein, unknown function QDO/A (1)  
PF3D7_0520800 conserved Plasmodium protein, unknown function TDO/A (1)  
PF3D7_0520900 S-adenosyl-L-homocysteine hydrolase (SAHH) TDO/A (1)  
PF3D7_0610100 Pre-mRNA-splicing factor SLU7, putative (SLU7) TDO/A (1)  
PF3D7_0611800 conserved Plasmodium protein, unknown function TDO/A (1)  
PF3D7_0613800 transcription factor with AP2 domain(s) (ApiAP2) QDO/A (1) RGVYF
TDO/A (1)
PF3D7_0623100 nuclear polyadenylated RNA-binding protein NAB2, putative TDO/A (2)  
PF3D7_0718100 exported serine/threonine protein kinase (EST) TDO/A (1)  
PF3D7_0720700 phosphoinositide-binding protein, putative TDO/A (1) KIKF
PF3D7_0724600 protein kinase, putative TDO/A (1)  
PF3D7_0803400 DNA repair protein rad54, putative QDO/A (1)  
PF3D7_0816600 ClpB protein, putative (ClpB1) TDO/A (1)  
PF3D7_0917900 heat shock protein 70 (HSP70-2) TDO/A (1)  
PF3D7_0919900 regulator of chromosome condensation, putative QDO/A (8) KSVSF
PF3D7_1008000 histone deacetylase 2 (HDA2) TDO/A (1)  
PF3D7_1008100 conserved Plasmodium protein, unknown function TDO/A (1) KSVSF
PF3D7_1020900 ADP-ribosylation factor (ARF1) TDO/A (1)  
PF3D7_1031600 conserved Plasmodium protein, unknown function (GEXP15) QDO/A (3) KKVQF
PF3D7_1105100 histone H2B (H2B) TDO/A (1)  
PF3D7_1130700 structural maintenance of chromosome protein, putative TDO/A (2)  
PF3D7_1202600 conserved protein, unknown function QDO/A (10) KNVTF/KCVSF/KQVTF/RTVSF/KKVTF/KSVSF/KKVTF
PF3D7_1205500 zinc finger protein, putative TDO/A (1)  
PF3D7_1228600 merozoite surface protein 9 (MSP9) TDO/A (1)  
PF3D7_1229600 conserved Plasmodium protein, unknown function TDO/A (1)  
PF3D7_1303800 conserved Plasmodium protein, unknown function TDO/A (1)  
PF3D7_1317600 conserved Plasmodium protein, unknown function TDO/A (1) KYIYF
PF3D7_1325800 conserved Plasmodium protein, unknown function TDO/A (1)  
PF3D7_1346100 protein transport protein SEC61 subunit alpha (SEC61) TDO/A (1) KGIEF
PF3D7_1358200 conserved Plasmodium protein, unknown function TDO/A (1)  
Out of frame genes mal_mito_2 cytochrome c oxidase subunit 1 (COX1) TDO/A (2)  
PF3D7_0110200 FAD-linked sulfhydryl oxidase ERV1, putative (ERV1) TDO/A (1) KINF
PF3D7_0202400 conserved Plasmodium protein, unknown function TDO/A (1)  
PF3D7_0205700.1 conserved Plasmodium protein, unknown function TDO/A (1)  
PF3D7_0207100 conserved Plasmodium protein, unknown function TDO/A (1)  
PF3D7_0207500 serine repeat antigen 6 (SERA6) TDO/A (1)  
PF3D7_0307700 conserved Plasmodium protein, unknown function TDO/A (1)  
PF3D7_0310400 parasite-infected erythrocyte surface protein (PIESP1) TDO/A (1)  
PF3D7_0312800 60S ribosomal protein L26, putative TDO/A (1)  
PF3D7_0317300 conserved Plasmodium protein, unknown function TDO/A (1)  
PF3D7_0319500 RNA binding protein, putative TDO/A (1)  
PF3D7_0407700 conserved Plasmodium protein, unknown function TDO/A (1) RDIIF
PF3D7_0417400 conserved Plasmodium protein, unknown function TDO/A (1)  
PF3D7_0418000 conserved Plasmodium protein, unknown function TDO/A (2)  
PF3D7_0418300 conserved Plasmodium protein, unknown function TDO/A (2)  
PF3D7_0419900 phosphatidylinositol 4-kinase, putative QDO/A (2)  
TDO/A (1)
PF3D7_0420000 zinc finger protein, putative TDO/A (1)  
PF3D7_0509200 leucine-rich repeat protein (LRR2) QDO/A (1)  
PF3D7_0516600 translation initiation factor IF-2 TDO/A (1)  
PF3D7_0516900 60S ribosomal protein L2 (RPL2) TDO/A (1) KVIF
PF3D7_0520800 conserved Plasmodium protein, unknown function QDO/A (1)  
PF3D7_0520900 S-adenosyl-L-homocysteine hydrolase (SAHH) TDO/A (1)  
PF3D7_0525200 conserved Plasmodium protein, unknown function TDO/A (2) KIEF/KDVLF
PF3D7_0530000 conserved Plasmodium protein, unknown function TDO/A (1)  
PF3D7_0532100 early transcribed membrane protein 5 (ETRAMP5) TDO/A (2)  
PF3D7_0602000 conserved Plasmodium protein, unknown function TDO/A (1)  
PF3D7_0604500 conserved Plasmodium protein, unknown function TDO/A (1)  
PF3D7_0611700 60S ribosomal protein L39 (RPL39) TDO/A (2)  
PF3D7_0613800 transcription factor with AP2 domain(s) (ApiAP2) TDO/A (1)  
PF3D7_0617200 conserved Plasmodium protein, unknown function QDO/A (1) KQIGF
PF3D7_0617800 histone H2A (H2A) QDO/A (2)  
TDO/A (6)
PF3D7_0625300 DNA polymerase 1, putative TDO/A (1)  
PF3D7_0709300 Cg2 protein (CG2) TDO/A (1) KVNF
PF3D7_0719900 conserved Plasmodium membrane protein, unknown function TDO/A (1)  
PF3D7_0721100 conserved Plasmodium protein, unknown function QDO/A (1)  
TDO/A (1)
PF3D7_0725300 conserved Plasmodium protein, unknown function TDO/A (1)  
PF3D7_0806900 conserved Plasmodium protein, unknown function QDO/A (1) KNIGF/KIRW
PF3D7_0809400 conserved Plasmodium protein, unknown function TDO/A (1)  
PF3D7_0811400 conserved protein, unknown function TDO/A (1)  
PF3D7_0813200 CS domain protein, putative TDO/A (1)  
PF3D7_0813300 conserved Plasmodium protein, unknown function TDO/A (1)  
PF3D7_0814000 60S ribosomal protein L13-2, putative QDO/A (2) RVNF
PF3D7_0815800 vacuolar sorting protein VPS9, putative TDO/A (1)  
PF3D7_0818000 conserved protein, unknown function TDO/A (1)  
PF3D7_0818900d heat shock protein 70 (HSP70) TDO/A (1)  
PF3D7_0831700 heat shock protein 70, putative (HSP70-x) QDO/A (1)  
PF3D7_0904000 GTPase-activating protein, putative TDO/A (1)  
PF3D7_0919900 regulator of chromosome condensation, putative TDO/A (1)  
PF3D7_0921700 conserved Plasmodium protein, unknown function QDO/A (1)  
PF3D7_0924100 conserved Plasmodium protein, unknown function TDO/A (1)  
PF3D7_0932100 protein MAM3, putative TDO/A (1)  
PF3D7_0933300 conserved Plasmodium protein, unknown function TDO/A (1)  
PF3D7_1020200 conserved Plasmodium protein, unknown function TDO/A (1) KNVFF
PF3D7_1023400 HORMA domain protein, putative QDO/A (1)  
PF3D7_1029900 conserved Plasmodium protein, unknown function TDO/A (1)  
PF3D7_1032000 ribosome maturation factor RimM, putative (RimM) TDO/A (1)  
PF3D7_1103100 60S acidic ribosomal protein P1, putative (RPP1) TDO/A (1)  
PF3D7_1105100 histone H2B (H2B) TDO/A (2)  
PF3D7_1107300 polyadenylate-binding protein-interacting protein 1, putative (PAIP1) TDO/A (1)  
PF3D7_1108600 endoplasmic reticulum-resident calcium binding protein (ERC) TDO/A (1) KVYF
PF3D7_1116200.1 pyridoxine biosynthesis protein PDX2 (PDX2) QDO/A (1)  
PF3D7_1117700 GTP-binding nuclear protein ran/tc4 (RAN) QDO/A (1) RQIQF
PF3D7_1121600 circumsporozoite-related antigen,exported protein 1 (EXP1) TDO/A (1)  
PF3D7_1127900 conserved Plasmodium protein, unknown function TDO/A (1)  
PF3D7_1141400 phosphatidylinositol N-acetylglucosaminyltransferase subunit H, putative (PIGH) TDO/A (1)  
PF3D7_1149000 antigen 332, DBL-like protein (Pf332) TDO/A (1) KIIW
PF3D7_1206800 conserved Plasmodium protein, unknown function QDO/A (1)  
PF3D7_1210600 conserved Plasmodium protein, unknown function QDO/A (1)  
PF3D7_1211200 conserved Plasmodium protein, unknown function TDO/A (1) KVKF/KRINF/KYIEF
PF3D7_1214100 GPI ethanolamine phosphate transferase 3, putative (PIGO) TDO/A (1)  
PF3D7_1222300 endoplasmin, putative (GRP94) TDO/A (1)  
PF3D7_1224000 GTP cyclohydrolase I (GCH1) TDO/A (1)  
PF3D7_1227000 conserved Plasmodium protein, unknown function TDO/A (2)  
PF3D7_1227700 conserved Plasmodium protein, unknown function TDO/A (1)  
PF3D7_1228600 merozoite surface protein 9 (MSP9) QDO/A (1)  
TDO/A (1)
PF3D7_1229400 macrophage migration inhibitory factor (MIF) QDO/A (1)  
TDO/A (1)
PF3D7_1231600 pre-mRNA-splicing factor ATP-dependent RNA helicase PRP2, putative (PRP2) TDO/A (1)  
PF3D7_1234600 conserved Plasmodium protein, unknown function TDO/A (1)  
PF3D7_1234900 conserved Plasmodium protein, unknown function TDO/A (1)  
PF3D7_1240600 erythrocyte membrane protein 1, PfEMP1 (VAR) TDO/A (1)  
PF3D7_1249800 THO complex subunit 2, putative (THO2) TDO/A (1)  
PF3D7_1303800 conserved Plasmodium protein, unknown function TDO/A (2)  
PF3D7_1306400 26S protease regulatory subunit 10B, putative (RPT4) TDO/A (1)  
PF3D7_1308400 conserved Plasmodium protein, unknown function QDO/A (1) RRVLF
PF3D7_1308800 tyrosine recombinase (INT) QDO/A (1) KYIKF
TDO/A (1)
PF3D7_1316100 inositol polyphosphate kinase, putative (IPK2) TDO/A (1)  
PF3D7_1324800 dihydrofolate synthase/folylpolyglutamate synthase (DHFS-FPGS) QDO/A (1)  
PF3D7_1326600 conserved Plasmodium protein, unknown function QDO/A (2)  
PF3D7_1336600 conserved Plasmodium protein, unknown function TDO/A (1)  
PF3D7_1342300 tetratricopeptide repeat family protein, putative TDO/A (1)  
PF3D7_1342900 transcription factor with AP2 domain(s) (ApiAP2) TDO/A (1)  
PF3D7_1346400 conserved Plasmodium protein, unknown function TDO/A (1)  
PF3D7_1348400 conserved Plasmodium membrane protein, unknown function TDO/A (1)  
PF3D7_1351200 conserved Plasmodium protein, unknown function TDO/A (1) KYVNW
PF3D7_1356900 protein kinase 5 (PK5) TDO/A (1)  
PF3D7_1357400 conserved Plasmodium protein, unknown function QDO/A (1) KSIRF
PF3D7_1360700 E3 SUMO-protein ligase PIAS, putative (PIAS) QDO/A (1) KKVLW
PF3D7_1361200 conserved Plasmodium protein, unknown function TDO/A (1)  
PF3D7_1366400 rhoptry protein (Rhop148) TDO/A (1) KILF
PF3D7_1417600 conserved Plasmodium protein, unknown function TDO/A (1)  
PF3D7_1423700 conserved Plasmodium protein, unknown function TDO/A (1)  
PF3D7_1444300 1-acyl-sn-glycerol-3-phosphate acyltransferase, putative (LPAAT) TDO/A (1)  
PF3D7_1456500 conserved Plasmodium protein, unknown function QDO/A (2)  
PF3D7_1457000 signal peptide peptidase (SPP) QDO/A (1)  
PF3D7_1460500 conserved Plasmodium protein, unknown function TDO/A (1) KVDF/KTVSF
PF3D7_1460700 60S ribosomal protein L27 (RPL27) TDO/A (1)  
PF3D7_1464500 conserved Plasmodium membrane protein, unknown function TDO/A (1)  
PF3D7_1465900 40S ribosomal protein S3, putative TDO/A (1)  
PF3D7_1466300 26S proteasome regulatory subunit RPN2, putative (RPN2) TDO/A (1)  
PF3D7_1470800 conserved Plasmodium protein, unknown function TDO/A (1)  
PF3D7_1471900 conserved Plasmodium protein, unknown function TDO/A (1) KIIEF
  1. aAnnotations available on PlasmoDB database (v24)
  2. bSelection medium used for the growth of isolated yeast clones were indicated. Numbers indicate the count of isolated clones. TDO/A Triple dropout supplemented with Aureobasidin A, QDO/A Quadruple dropout supplemented with Aureobasidin A
  3. cThe presence and the sequence of RVxF binding motifs were indicated
  4. dShared proteins isolated by affinity purification and Y2H screening