Analysis of the interactome of the Ser/Thr Protein Phosphatase type 1 in Plasmodium falciparum

Hollin, Thomas; De Witte, Caroline; Lenne, Astrid; Pierrot, Christine; Khalife, Jamal

doi:10.1186/s12864-016-2571-z

BMC Genomics

Table 2 Pips identified by Yeast two-hybrid screening

From: Analysis of the interactome of the Ser/Thr Protein Phosphatase type 1 in Plasmodium falciparum

	PlasmoDB Accession number^a	Name^a	Growth on selective plates (number of isolated clones)^b	RVxF motifs^c
In frame genes	PF3D7_0107600	serine/threonine protein kinase, putative	TDO/A (1)
	PF3D7_0220000	liver stage antigen 3 (LSA3)	QDO/A (2)	KKVRF
	PF3D7_0418300	conserved Plasmodium protein, unknown function	QDO/A (1)
	PF3D7_0520800	conserved Plasmodium protein, unknown function	TDO/A (1)
	PF3D7_0520900	S-adenosyl-L-homocysteine hydrolase (SAHH)	TDO/A (1)
	PF3D7_0610100	Pre-mRNA-splicing factor SLU7, putative (SLU7)	TDO/A (1)
	PF3D7_0611800	conserved Plasmodium protein, unknown function	TDO/A (1)
	PF3D7_0613800	transcription factor with AP2 domain(s) (ApiAP2)	QDO/A (1)	RGVYF
	PF3D7_0613800	transcription factor with AP2 domain(s) (ApiAP2)	TDO/A (1)	RGVYF
	PF3D7_0623100	nuclear polyadenylated RNA-binding protein NAB2, putative	TDO/A (2)
	PF3D7_0718100	exported serine/threonine protein kinase (EST)	TDO/A (1)
	PF3D7_0720700	phosphoinositide-binding protein, putative	TDO/A (1)	KIKF
	PF3D7_0724600	protein kinase, putative	TDO/A (1)
	PF3D7_0803400	DNA repair protein rad54, putative	QDO/A (1)
	PF3D7_0816600	ClpB protein, putative (ClpB1)	TDO/A (1)
	PF3D7_0917900	heat shock protein 70 (HSP70-2)	TDO/A (1)
	PF3D7_0919900	regulator of chromosome condensation, putative	QDO/A (8)	KSVSF
	PF3D7_1008000	histone deacetylase 2 (HDA2)	TDO/A (1)
	PF3D7_1008100	conserved Plasmodium protein, unknown function	TDO/A (1)	KSVSF
	PF3D7_1020900	ADP-ribosylation factor (ARF1)	TDO/A (1)
	PF3D7_1031600	conserved Plasmodium protein, unknown function (GEXP15)	QDO/A (3)	KKVQF
	PF3D7_1105100	histone H2B (H2B)	TDO/A (1)
	PF3D7_1130700	structural maintenance of chromosome protein, putative	TDO/A (2)
	PF3D7_1202600	conserved protein, unknown function	QDO/A (10)	KNVTF/KCVSF/KQVTF/RTVSF/KKVTF/KSVSF/KKVTF
	PF3D7_1205500	zinc finger protein, putative	TDO/A (1)
	PF3D7_1228600	merozoite surface protein 9 (MSP9)	TDO/A (1)
	PF3D7_1229600	conserved Plasmodium protein, unknown function	TDO/A (1)
	PF3D7_1303800	conserved Plasmodium protein, unknown function	TDO/A (1)
	PF3D7_1317600	conserved Plasmodium protein, unknown function	TDO/A (1)	KYIYF
	PF3D7_1325800	conserved Plasmodium protein, unknown function	TDO/A (1)
	PF3D7_1346100	protein transport protein SEC61 subunit alpha (SEC61)	TDO/A (1)	KGIEF
	PF3D7_1358200	conserved Plasmodium protein, unknown function	TDO/A (1)
Out of frame genes	mal_mito_2	cytochrome c oxidase subunit 1 (COX1)	TDO/A (2)
	PF3D7_0110200	FAD-linked sulfhydryl oxidase ERV1, putative (ERV1)	TDO/A (1)	KINF
	PF3D7_0202400	conserved Plasmodium protein, unknown function	TDO/A (1)
	PF3D7_0205700.1	conserved Plasmodium protein, unknown function	TDO/A (1)
	PF3D7_0207100	conserved Plasmodium protein, unknown function	TDO/A (1)
	PF3D7_0207500	serine repeat antigen 6 (SERA6)	TDO/A (1)
	PF3D7_0307700	conserved Plasmodium protein, unknown function	TDO/A (1)
	PF3D7_0310400	parasite-infected erythrocyte surface protein (PIESP1)	TDO/A (1)
	PF3D7_0312800	60S ribosomal protein L26, putative	TDO/A (1)
	PF3D7_0317300	conserved Plasmodium protein, unknown function	TDO/A (1)
	PF3D7_0319500	RNA binding protein, putative	TDO/A (1)
	PF3D7_0407700	conserved Plasmodium protein, unknown function	TDO/A (1)	RDIIF
	PF3D7_0417400	conserved Plasmodium protein, unknown function	TDO/A (1)
	PF3D7_0418000	conserved Plasmodium protein, unknown function	TDO/A (2)
	PF3D7_0418300	conserved Plasmodium protein, unknown function	TDO/A (2)
	PF3D7_0419900	phosphatidylinositol 4-kinase, putative	QDO/A (2)
	PF3D7_0419900	phosphatidylinositol 4-kinase, putative	TDO/A (1)
	PF3D7_0420000	zinc finger protein, putative	TDO/A (1)
	PF3D7_0509200	leucine-rich repeat protein (LRR2)	QDO/A (1)
	PF3D7_0516600	translation initiation factor IF-2	TDO/A (1)
	PF3D7_0516900	60S ribosomal protein L2 (RPL2)	TDO/A (1)	KVIF
	PF3D7_0520800	conserved Plasmodium protein, unknown function	QDO/A (1)
	PF3D7_0520900	S-adenosyl-L-homocysteine hydrolase (SAHH)	TDO/A (1)
	PF3D7_0525200	conserved Plasmodium protein, unknown function	TDO/A (2)	KIEF/KDVLF
	PF3D7_0530000	conserved Plasmodium protein, unknown function	TDO/A (1)
	PF3D7_0532100	early transcribed membrane protein 5 (ETRAMP5)	TDO/A (2)
	PF3D7_0602000	conserved Plasmodium protein, unknown function	TDO/A (1)
	PF3D7_0604500	conserved Plasmodium protein, unknown function	TDO/A (1)
	PF3D7_0611700	60S ribosomal protein L39 (RPL39)	TDO/A (2)
	PF3D7_0613800	transcription factor with AP2 domain(s) (ApiAP2)	TDO/A (1)
	PF3D7_0617200	conserved Plasmodium protein, unknown function	QDO/A (1)	KQIGF
	PF3D7_0617800	histone H2A (H2A)	QDO/A (2)
	PF3D7_0617800	histone H2A (H2A)	TDO/A (6)
	PF3D7_0625300	DNA polymerase 1, putative	TDO/A (1)
	PF3D7_0709300	Cg2 protein (CG2)	TDO/A (1)	KVNF
	PF3D7_0719900	conserved Plasmodium membrane protein, unknown function	TDO/A (1)
	PF3D7_0721100	conserved Plasmodium protein, unknown function	QDO/A (1)
	PF3D7_0721100	conserved Plasmodium protein, unknown function	TDO/A (1)
	PF3D7_0725300	conserved Plasmodium protein, unknown function	TDO/A (1)
	PF3D7_0806900	conserved Plasmodium protein, unknown function	QDO/A (1)	KNIGF/KIRW
	PF3D7_0809400	conserved Plasmodium protein, unknown function	TDO/A (1)
	PF3D7_0811400	conserved protein, unknown function	TDO/A (1)
	PF3D7_0813200	CS domain protein, putative	TDO/A (1)
	PF3D7_0813300	conserved Plasmodium protein, unknown function	TDO/A (1)
	PF3D7_0814000	60S ribosomal protein L13-2, putative	QDO/A (2)	RVNF
	PF3D7_0815800	vacuolar sorting protein VPS9, putative	TDO/A (1)
	PF3D7_0818000	conserved protein, unknown function	TDO/A (1)
	PF3D7_0818900^d	heat shock protein 70 (HSP70)	TDO/A (1)
	PF3D7_0831700	heat shock protein 70, putative (HSP70-x)	QDO/A (1)
	PF3D7_0904000	GTPase-activating protein, putative	TDO/A (1)
	PF3D7_0919900	regulator of chromosome condensation, putative	TDO/A (1)
	PF3D7_0921700	conserved Plasmodium protein, unknown function	QDO/A (1)
	PF3D7_0924100	conserved Plasmodium protein, unknown function	TDO/A (1)
	PF3D7_0932100	protein MAM3, putative	TDO/A (1)
	PF3D7_0933300	conserved Plasmodium protein, unknown function	TDO/A (1)
	PF3D7_1020200	conserved Plasmodium protein, unknown function	TDO/A (1)	KNVFF
	PF3D7_1023400	HORMA domain protein, putative	QDO/A (1)
	PF3D7_1029900	conserved Plasmodium protein, unknown function	TDO/A (1)
	PF3D7_1032000	ribosome maturation factor RimM, putative (RimM)	TDO/A (1)
	PF3D7_1103100	60S acidic ribosomal protein P1, putative (RPP1)	TDO/A (1)
	PF3D7_1105100	histone H2B (H2B)	TDO/A (2)
	PF3D7_1107300	polyadenylate-binding protein-interacting protein 1, putative (PAIP1)	TDO/A (1)
	PF3D7_1108600	endoplasmic reticulum-resident calcium binding protein (ERC)	TDO/A (1)	KVYF
	PF3D7_1116200.1	pyridoxine biosynthesis protein PDX2 (PDX2)	QDO/A (1)
PF3D7_1117700	GTP-binding nuclear protein ran/tc4 (RAN)	QDO/A (1)	RQIQF
PF3D7_1121600	circumsporozoite-related antigen,exported protein 1 (EXP1)	TDO/A (1)
PF3D7_1127900	conserved Plasmodium protein, unknown function	TDO/A (1)
PF3D7_1141400	phosphatidylinositol N-acetylglucosaminyltransferase subunit H, putative (PIGH)	TDO/A (1)
PF3D7_1149000	antigen 332, DBL-like protein (Pf332)	TDO/A (1)	KIIW
PF3D7_1206800	conserved Plasmodium protein, unknown function	QDO/A (1)
PF3D7_1210600	conserved Plasmodium protein, unknown function	QDO/A (1)
PF3D7_1211200	conserved Plasmodium protein, unknown function	TDO/A (1)	KVKF/KRINF/KYIEF
PF3D7_1214100	GPI ethanolamine phosphate transferase 3, putative (PIGO)	TDO/A (1)
PF3D7_1222300	endoplasmin, putative (GRP94)	TDO/A (1)
PF3D7_1224000	GTP cyclohydrolase I (GCH1)	TDO/A (1)
PF3D7_1227000	conserved Plasmodium protein, unknown function	TDO/A (2)
PF3D7_1227700	conserved Plasmodium protein, unknown function	TDO/A (1)
PF3D7_1228600	merozoite surface protein 9 (MSP9)	QDO/A (1)
PF3D7_1228600	merozoite surface protein 9 (MSP9)	TDO/A (1)
PF3D7_1229400	macrophage migration inhibitory factor (MIF)	QDO/A (1)
PF3D7_1229400	macrophage migration inhibitory factor (MIF)	TDO/A (1)
PF3D7_1231600	pre-mRNA-splicing factor ATP-dependent RNA helicase PRP2, putative (PRP2)	TDO/A (1)
PF3D7_1234600	conserved Plasmodium protein, unknown function	TDO/A (1)
PF3D7_1234900	conserved Plasmodium protein, unknown function	TDO/A (1)
PF3D7_1240600	erythrocyte membrane protein 1, PfEMP1 (VAR)	TDO/A (1)
PF3D7_1249800	THO complex subunit 2, putative (THO2)	TDO/A (1)
PF3D7_1303800	conserved Plasmodium protein, unknown function	TDO/A (2)
PF3D7_1306400	26S protease regulatory subunit 10B, putative (RPT4)	TDO/A (1)
PF3D7_1308400	conserved Plasmodium protein, unknown function	QDO/A (1)	RRVLF
PF3D7_1308800	tyrosine recombinase (INT)	QDO/A (1)	KYIKF
PF3D7_1308800	tyrosine recombinase (INT)	TDO/A (1)	KYIKF
PF3D7_1316100	inositol polyphosphate kinase, putative (IPK2)	TDO/A (1)
PF3D7_1324800	dihydrofolate synthase/folylpolyglutamate synthase (DHFS-FPGS)	QDO/A (1)
PF3D7_1326600	conserved Plasmodium protein, unknown function	QDO/A (2)
PF3D7_1336600	conserved Plasmodium protein, unknown function	TDO/A (1)
PF3D7_1342300	tetratricopeptide repeat family protein, putative	TDO/A (1)
PF3D7_1342900	transcription factor with AP2 domain(s) (ApiAP2)	TDO/A (1)
PF3D7_1346400	conserved Plasmodium protein, unknown function	TDO/A (1)
PF3D7_1348400	conserved Plasmodium membrane protein, unknown function	TDO/A (1)
PF3D7_1351200	conserved Plasmodium protein, unknown function	TDO/A (1)	KYVNW
PF3D7_1356900	protein kinase 5 (PK5)	TDO/A (1)
PF3D7_1357400	conserved Plasmodium protein, unknown function	QDO/A (1)	KSIRF
PF3D7_1360700	E3 SUMO-protein ligase PIAS, putative (PIAS)	QDO/A (1)	KKVLW
PF3D7_1361200	conserved Plasmodium protein, unknown function	TDO/A (1)
PF3D7_1366400	rhoptry protein (Rhop148)	TDO/A (1)	KILF
PF3D7_1417600	conserved Plasmodium protein, unknown function	TDO/A (1)
PF3D7_1423700	conserved Plasmodium protein, unknown function	TDO/A (1)
PF3D7_1444300	1-acyl-sn-glycerol-3-phosphate acyltransferase, putative (LPAAT)	TDO/A (1)
PF3D7_1456500	conserved Plasmodium protein, unknown function	QDO/A (2)
PF3D7_1457000	signal peptide peptidase (SPP)	QDO/A (1)
PF3D7_1460500	conserved Plasmodium protein, unknown function	TDO/A (1)	KVDF/KTVSF
PF3D7_1460700	60S ribosomal protein L27 (RPL27)	TDO/A (1)
PF3D7_1464500	conserved Plasmodium membrane protein, unknown function	TDO/A (1)
PF3D7_1465900	40S ribosomal protein S3, putative	TDO/A (1)
PF3D7_1466300	26S proteasome regulatory subunit RPN2, putative (RPN2)	TDO/A (1)
PF3D7_1470800	conserved Plasmodium protein, unknown function	TDO/A (1)
PF3D7_1471900	conserved Plasmodium protein, unknown function	TDO/A (1)	KIIEF

^aAnnotations available on PlasmoDB database (v24)
^bSelection medium used for the growth of isolated yeast clones were indicated. Numbers indicate the count of isolated clones. TDO/A Triple dropout supplemented with Aureobasidin A, QDO/A Quadruple dropout supplemented with Aureobasidin A
^cThe presence and the sequence of RVxF binding motifs were indicated
^dShared proteins isolated by affinity purification and Y2H screening

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