Skip to main content
Fig. 3 | BMC Genomics

Fig. 3

From: Deciphering the transcriptional regulation and spatiotemporal distribution of immunity response in barley to Pyrenophora graminea fungal invasion

Fig. 3

Temporal expression of ESTs (genes) selected by RT-PCR differential screening. After extraction of total RNA from plant tissues at 14 and 20 dpi, reverse-transcripts were produced for carrying-out qPCR analysis. Control to normalize amplification was run with the Ef1-╬▒ specific primers. Transcripts were analyzed using comparative Ct method. Data are presented in color scales: five shades of red from dark red to light red for upregulated genes and five shades of green for the downregulated genes. Non-differential expression is mentioned in black. The value of relative quantification (RQ) from qPCR was represented by the ladder of both colors (top panel)

Back to article page