Fig. 8

Elevated callose deposition in resistant genotype prevents Pg fungal growth but not susceptible genotype. Tests were performed at 14 dpi. a Leaves were stained with aniline blue to visualize callose deposition by blue fluorescence using florescence microscopy (upper panel). Relative fluorescence intensity emitted by aniline blue-stained callose depositions (CD) was calculated (lower panel) on photographs taken under UV filter. The average of 10 tissue samples of each category is presented as a percentage. Scale bar = 20 μm. Samples with mycelium and second hyphae (or control tissues) were also washed off, stained by trypan blue and passed on optical microscope to visualize the presence of fungus. b Quantification of fungal Pg DNA presence in roots and shoots of resistant and susceptible plants inoculated in liquid culture for 10 days was performed using qPCR. Student’s t test was applied and asterisks indicate a statistical difference at P < 0.001 on each sample mean. The error bars correspond to the 95 % confidence interval calculated from the test