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Table 5 Genes validated between qRT-PCR and RNAseq methodologies, including fold change (FC), P-values and correlation coefficients (R)

From: Effect of dietary restriction and subsequent re-alimentation on the transcriptional profile of hepatic tissue in cattle

  RNAseq RT-qPCR Correlation
Gene FC P-Value FC P-Value R P-Value
Period 1       
SOCS3 −1.17 0.244 −0.61 0.0924 0.59 0.052
JAK2 1.15 0.227 0.71 0.602 0.61 0.044
STAT5B −1.07 0.576 −0.69 1 0.57 0.031
IGF1 −2.53 <.0001 −1.06 0.001 0.48 0.013
IGFBP1 3.62 <.0001 3.91 <.0001 0.95 <0.001
IGFBP2 29.25 <.0001 8.45 <.0001 0.52 0.008
IGFBP3 −1.11 0.139 −0.84 0.684 0.63 0.045
IGFBP4 1.01 0.952 0.65 0.872 0.51 0.094
IGFBP5 −1.48 0.401 −1.13 0.999 0.70 0.012
IGFBP6 1.83 <.0001 1.74 <.0001 0.65 0.023
ALS 1.05 0.641 1.02 0.648 0.82 0.037
GHR −1.89 <.0001 −1.39 0.0274 0.77 0.048
Period 2       
SOCS3 1.05 0.722 0.065 0.963 0.49 0.089
JAK2 1.13 0.339 0.75 0.641 0.63 0.054
STAT5B 1.09 0.466 0.62 0.999 0.48 0.036
IGF1 −1.01 0.914 −0.76 0.995 0.72 0.037
IGFBP1 1.29 0.032 1.00 0.043 0.81 0.002
IGFBP2 1.04 0.755 0.51 0.910 0.59 0.054
IGFBP3 1.05 0.669 0.58 0.979 0.29 0.068
IGFBP4 −1.04 0.731 −0.83 0.993 0.42 0.047
IGFBP5 1.33 0.051 1.00 0.495 0.16 0.054
IGFBP6 −1.23 0.625 −0.77 0.990 0.57 0.024
ALS −1.01 0.955 −0.88 1 0.49 0.078
GHR 1.03 0.048 1.09 0.041 0.86 0.025