Table 5 Genes validated between qRT-PCR and RNAseq methodologies, including fold change (FC), P-values and correlation coefficients (R)

Period 1

SOCS3

−1.17

0.244

−0.61

0.0924

0.59

0.052

JAK2

1.15

0.227

0.71

0.602

0.61

0.044

STAT5B

−1.07

0.576

−0.69

1

0.57

0.031

IGF1

−2.53

<.0001

−1.06

0.001

0.48

0.013

IGFBP1

3.62

<.0001

3.91

<.0001

0.95

<0.001

IGFBP2

29.25

<.0001

8.45

<.0001

0.52

0.008

IGFBP3

−1.11

0.139

−0.84

0.684

0.63

0.045

IGFBP4

1.01

0.952

0.65

0.872

0.51

0.094

IGFBP5

−1.48

0.401

−1.13

0.999

0.70

0.012

IGFBP6

1.83

<.0001

1.74

<.0001

0.65

0.023

ALS

1.05

0.641

1.02

0.648

0.82

0.037

GHR

−1.89

<.0001

−1.39

0.0274

0.77

0.048

Period 2

SOCS3

1.05

0.722

0.065

0.963

0.49

0.089

JAK2

1.13

0.339

0.75

0.641

0.63

0.054

STAT5B

1.09

0.466

0.62

0.999

0.48

0.036

IGF1

−1.01

0.914

−0.76

0.995

0.72

0.037

IGFBP1

1.29

0.032

1.00

0.043

0.81

0.002

IGFBP2

1.04

0.755

0.51

0.910

0.59

0.054

IGFBP3

1.05

0.669

0.58

0.979

0.29

0.068

IGFBP4

−1.04

0.731

−0.83

0.993

0.42

0.047

IGFBP5

1.33

0.051

1.00

0.495

0.16

0.054

IGFBP6

−1.23

0.625

−0.77

0.990

0.57

0.024

ALS

−1.01

0.955

−0.88

1

0.49

0.078

GHR

1.03

0.048

1.09

0.041

0.86

0.025