Fig. 4

GSEA analysis comparing the gene profile of cAMP-treated astrocytes with the transcriptome signatures of astroglial cells in different states. a The upregulated gene set identified in cAMP-treated astrocytes is significantly enriched in mature and in vivo astroglial cells, while the cAMP-induced downregulated gene set is significantly enriched in developing, in vitro, proinflammatory cytokine-treated astrocytes and after MCAO and LPS treatment. FDR q-value, False discovery rate; NES, Normalized Enrichment Score. b Representative GSEA analysis showing the plots of cAMP-regulated gene profile with in vivo (n = 2821 genes) and in vitro (n = 2105 genes) gene sets obtained from Cahoy et al. 2008. Signal-to-Noise ratio statistic was used to rank the genes on the basis of their correlation with either the upregulation (red) or downregulation (blue). On each panel, the vertical black lines indicate the position of each gene of the set in the ordered, non-redundant data set. The green curve corresponds to the enrichment profile curve, which is the running sum of the weighted enrichment score obtained with GSEA software. c Proportional Venn diagrams representing the overlap of astroglial genes differentially expressed by cAMP compared to the gene sets in different astrocyte states (fold change cut-off 1.5 in each case except for LPS and MCAO that fold-change cut off was 4)