Fig. 2

Finding Missing IMG Terms Using Function Profile. A user first selects an IMG Part List Nodulation factor biosynthesis, export and regulation to load all component IMG terms into Function Cart (Fig. 2 (i)). All Bradyrhizobium genomes are supposed to have genes associated with these terms. However, some terms are missing in certain genomes (Fig. 2 (ii)). Clicking on the zero count will lead to searching potential genes using BLAST as the result shown in Fig. 2 (iii). Since microbial genes with related functions tend to be close together on the scaffold, an alternative approach is to investigate intergenic regions of genes with functions to look for potential missing genes (Fig. 2 (iv))