Fig. 8

Biosynthetic Cluster and Secondary Metabolite Annotation. Experimentally verified biosynthetic clusters are associated with secondary metabolites, while such information is missing from predicted biosynthetic clusters. Biosynthetic cluster 160962703 of Streptomyces sp. WT1 is an experimentally verified cluster (Genbank Accession JN207130) associated with natural product Mevalonate. Genes of this cluster participate in 7 KEGG modules (Fig. 8 (i)). KEGG Module Map M00095 C5 isoprenoid biosynthesis, mevalonate pathway for this cluster shows that genes in this cluster are linked to 6 of the KO terms (Fig. 8 (ii)). Predicted biosynthetic cluster 161507570 of Streptomyces fradiae ATCC 19609 does not have any secondary metabolite information. However, it contains 6 genes associated with the same 6 KO terms of M00095, which is a good indication that the cluster can produce the same secondary metabolite (Fig. 8 (iii)). Users can use the “Add SM Annotation” function to annotate the association (Fig. 8 (iv))