Fig. 3

miRNA expression across regenerating tail sections by qRT-PCR. Total RNA was extracted from regenerated tail sections from four biological replicates. cDNA was synthesized using primers specific to the mature miRNA. Three qRT-PCR replicates were performed for each gene in each tail section (600 total reactions). Expression is normalized to ef1a, and quantified using the ∆∆Ct method. a Highly conserved miRNAs with expression decreasing from the base to the tip. b Highly conserved miRNAs with expression increasing from the base to the tip. c Expression of putative novel miRNAs across regenerating tail sections