Library
|
Total read depth
|
Allele frequency
|
---|
1
|
38,000
|
0.0042
|
2
|
38,000
|
0.0025
|
3
|
71,000
|
0.0022
|
4
|
66,000
|
0.0020
|
- Four control “spike-in” DNA mixtures were created by serial dilution of one E. coli substrain (W3110) into another (Rel606). The libraries were PELE-sequenced to an average total read depth of 53,000× OPE. The rare alleles detected in the control libraries had average allele frequencies ranging from 0.20–0.42 % or 1/238-1/500 of total reads