Average read depth per barcode
|
PELE positives
|
PELE false positives
|
ORP positives
|
ORP false positives
|
Standard positives
|
Standard false positives
|
---|
1000
|
13
|
0
|
6
|
0
|
6
|
2
|
5000
|
19
|
0
|
18
|
0
|
24
|
7
|
10000
|
42
|
0
|
37
|
0
|
36
|
12
|
15000
|
36
|
0
|
32
|
0
|
35
|
13
|
18000
|
40
|
0
|
35
|
0
|
41
|
42
|
- A control spike-in library containing 64 expected rare alleles present at 0.42 % frequency was sequenced with the PELE-Seq, ORP, and Standard DNA-Seq methods at various read depths. The read depths listed are for the overlapping paired-end (OPE) reads per barcode of the PELE-Seq libraries. The methods are compared using the same number of raw reads, such that the standard DNA-Seq bam files have a read depth that is 2.4× that of the PELE-Seq bam files (2400–43,000× per barcode), to account for the loss associated with merging overlapped reads to create ORPs