Fig. 2
Salmonella LPS induced acute thymocyte death in chicks. Newly hatched chicks were injected i.p. with saline or 50 mg/kg Salmonella LPS and then sacrificed at defined time points (n = 3 ~ 6) to analyze cell death by the formamide-MAb assay and TUNEL assay. The positive cells were mainly distributed in thymic cortex. At least 5 fields in each section of the thymus were sampled, and positive cells per 1 × 105 μm2 in the thymic cortex were quantified. Scale bars = 50 μm. Data are presented as means ± SD. Statistically significant differences between LPS and saline groups at each time point were determined using Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001