Fig. 2
From: Enhanced whole exome sequencing by higher DNA insert lengths
Target regions and relative read coverages. a Agilent SureSelectXT v5+UTR target regions (75 Mb) consisted of 68 % overlapping bases to v5 and a unique fraction of 32 %. The target region of v5 (50 Mb) was nearly fully contained in v5+UTR. b Mean coverage of unmerged and merged paired-end reads considering the size of respective target regions 75 and 50 Mbp for 130 bp and 170 bp, respectively. The average coverage was higher in 130 bp inserts than in 170 bp. This difference declined substantially when merging joint paired-end reads. c Recalculation of coverage of unmerged and merged paired-end reads on the respective specific target regions and on common target regions only. The mean coverage on the respective target regions was higher in 130 bp insert samples. d Portion of respective target regions covered by at least 10 ×. Despite higher coverage means for 130 bp, a smaller fraction of target regions was apparent at ≥10× depth for 130 bp samples. For overlapping target regions of v5 and v5+UTR the fraction of covered regions was still not higher in 130 bp reads as would be implicated by the higher coverage means