Fig. 4

ARACNe network (Network 2) of all seasonally-biased genes, specifying core immune genes and timeless as hubs. Nodes, shown in a modified force-directed layout, represent gene module sizes associated with hubs and edges are Jaccard similarity coefficients for module composition (cut-off, 0.1). Modules associated with winter and summer-biased immune hubs segregate to different regions of the network, with the strongest winter-summer module similarity between orai1 and cd8a. The module associated with the summer-biased timeless is primarily similar to modules associated with winter-biased hubs. For larger modules the heat map (bottom left) shows significant odds ratio gene overlaps with gene sets representing organismal signatures of metabolism, growth, reproduction and stress