Host-induced aneuploidy and phenotypic diversification in the Sudden Oak Death pathogen Phytophthora ramorum

BMC Genomics

Table 1 Summary of phenotypic conversion upon passage experiments

Inoculated host, year	# Trees (# Leaves)^a	Inoculum; duration in host	Phenotypes^f		Total
Inoculated host, year	# Trees (# Leaves)^a	Inoculum; duration in host	wt	nwt	Total
Petri plate control	n/a	Pr-710, Pr-745, Pr-1556, Pr-1557, ND886 (wt, foliar hosts)^b ; n/a	348	0	348
Petri plate control	n/a	Pr-102 (nwt, oak)^c ; n/a	71	29	100
Canyon live oak, 2010	8	Pr-710 and Pr-745 (wt, bay); 20 weeks	37	25	62
Canyon live oak, 2010	10	Pr-710 and Pr-745 (wt, bay); 40 weeks	23	35	58
Shreve oak, 2010	2	Pr-710 and Pr-745 (wt, bay); 20 weeks	6	2	8
Canyon live oak, 2012	4	Pr-1556 and Pr-1557 (wt, bay); 20 weeks	27^d	0	27
California bay, 2012	1 (9)	Pr-745 (wt, bay); 20 weeks	9	0	9
California bay, 2013	3 (27)	ND886 (wt, camellia); 4 to 25 weeks^e	20	0	20
California bay, 2013	3 (27)	Pr-102 (nwt, oak); 4 to 25 weeks^e	16	2	18

^aThe total number of trees and the total number of leaves re-isolation were attempted. The pathogen was inoculated on oak stems or California bay leaves
^bcolony morphology of five wt isolates grown on Petri plates was scored. 24, 24, 100, 100 and 100 replicates were made for Pr-710, Pr-745, Pr-1556, Pr-1557, and ND886, respectively
^cPr-102 displaying wt colony morphology was used as inoculum. The 71 subcultures showing wt morphology were expected to display nwt colony morphology at a certain percentage upon subsequent subculturing
^dMost of the tested wt isolates displayed nwt after passage through race tubes
^ere-isolation was made 4, 12 and 25 weeks post-inoculation
^fCriteria for scoring of wt/nwt are described in Evaluation of colony morphology in Methods

ISSN: 1471-2164