Fig. 1

The effects of Aβ(1–42) peptide on 5hmC level in vitro. a-b Dot-blot assay shows Aβ treatment significantly decreased total 5hmC levels in a dose-dependent manner in HEK293ft cells. Aβ at 1-μM concentration is the most effective at decreasing 5hmC levels (*p < 0.05;** p < 0.01, unpaired t-test). c-f The cultured aNSCs are positive for neural stem cell markers Nestin (d) and SOX2 (e). g The representative images of dot-blot assay of Aβ treatment on 5hmC level in aNSCs. h The quantification result indicates Aβ at 1-μM concentration significantly decreases 5hmC level in aNSCs (*p < 0.05;** p < 0.01, unpaired t-test). i-j Dot-blot assay indicates that the global level of 5hmC in cortex is significantly higher than in HEK293ft cells and aNSCs (*p < 0.05;** p < 0.01, unpaired t-test)