Skip to main content
Fig. 1 | BMC Genomics

Fig. 1

From: The essential genome of Streptococcus agalactiae

Fig. 1

pCAM48 map. pCAM48 is a temperature-sensitive shuttle vector for delivery of a Himar1 mini-transposon, flanked by inverted repeat (IR) sequences modified to contain MmeI restriction enzyme sites. An erythromycin resistance marker (Ermr) is included within the transposon, as is the R6kγ origin of replication (Ori R6kγ), which can be used for plasmid rescue (not employed in this study). Outside of the mini-transposon, the vector contains a ColE1 origin of replication, the Gram-positive temperature-sensitive replicase RepA TS, in which the single-bp deletion present in pCAM45 has been repaired, and a kanamycin resistance marker (Kanr). The C9 Himar1 transposase gene is under the control of the S. pyogenes M1 gyrA promoter. Restriction enzyme sites used in development and analysis of pCAM48 are labeled

Back to article page