Fig. 1

pCAM48 map. pCAM48 is a temperature-sensitive shuttle vector for delivery of a Himar1 mini-transposon, flanked by inverted repeat (IR) sequences modified to contain MmeI restriction enzyme sites. An erythromycin resistance marker (Erm^r) is included within the transposon, as is the R6kγ origin of replication (Ori R6kγ), which can be used for plasmid rescue (not employed in this study). Outside of the mini-transposon, the vector contains a ColE1 origin of replication, the Gram-positive temperature-sensitive replicase RepA TS, in which the single-bp deletion present in pCAM45 has been repaired, and a kanamycin resistance marker (Kan^r). The C9 Himar1 transposase gene is under the control of the S. pyogenes M1 gyrA promoter. Restriction enzyme sites used in development and analysis of pCAM48 are labeled