Fig. 2

Transposon mutant library metrics. Sequential pooling of data from four T₀ sequencing runs (for libraries A₂ pilot, A₂ repeat, A₅, and A₇) demonstrates increasing unique insertion counts per gene (a). Library transposon saturation rates (percent of unique TA sites with an insertion) are shown for pilot and pooled datasets (b). ESSENTIALS plots of a kernel density function of log transformed measured vs. expected transposon insertion ratios show the expected bimodal distribution separating essential from nonessential genes using pooled libraries. Local minima values were used as determinants of gene essentiality at each time point (c). Correlation of library A₂ log₂ FC values for each gene from the pilot and technical replicate experiments for each time point, and between the three pairs of biological triplicate libraries for T₀. Pearson r values and two-tailed P values are listed (d)