The stepwise evolution of the exome during acquisition of docetaxel resistance in breast cancer cells

BMC Genomics

Table 1 Evolution of docetaxel resistance in MCF-7 cells

Docetaxel concentration	Passage numbers	Days at given concentration^a	Subpopulation for analysis	Doubling time (Days)
0 (Parental cells)	-	-	MCF-7_PAR	1.3
10 pM	1–3	12
30 pM	4–6	13
90 pM	7–9	18
270 pM	10–12	17	MCF-7_SUB-0.27nM	1.9
810 pM	13–15	12
1.2 nM	16–18	69	MCF-7_SUB-1.2nM	1.8
1.8 nM	19–21	25
2.25 nM	22–24	13
2.8 nM	25–27	19
3.5 nM	28–30	20
5 nM	31–33	18
7.5 nM	34–36	12	MCF-7_SUB-7.5nM	1.9
15 nM	37–39	13
30 nM	40–42	16
45 nM	43–45	24	MCF-7_SUB-45nM	1.8
55 nM	46–48	26
65 nM	49-		MCF-7_RES-65nM	1.4

^aNumbers in bold indicate concentration levels at which the cells took more than the average 20 days per concentration step to recover and go through 3 passages

ISSN: 1471-2164