Fig. 5

Expression of wheat annexin genes in response to abiotic stress and ABA by real-time PCR. Wheat Jinghua 9 seedlings grown for 14 days on MS medium were treated with PEG-6000 (25 % w/v) (a) 200 ummol · L⁻¹ ABA (b) 4 °C (c) and 250 mM NaCl (d) for 0, 1, 2, 5, 10 and 24 h, respectively. Total RNA was extracted from Jinghua 9 seedlings and used for real-time PCR. The expression level of wheat actin was used as the internal control to standardize the RNA samples for each reaction, and the expression in the treated 0 h samples was set as 1. Each data point is the average of three biological repeats, and error bars represent the standard error