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Fig. 1 | BMC Genomics

Fig. 1

From: Quantitation of next generation sequencing library preparation protocol efficiencies using droplet digital PCR assays - a systematic comparison of DNA library preparation kits for Illumina sequencing

Fig. 1

Principle of ddPCR – the droplet generator creates an emulsion with the sample containing the DNA, PCR enzyme and buffer, specific primers and Taqman probe (left). Only droplets containing a DNA fragment will exhibit a high fluorescence after the PCR amplification (middle). The sample is then analysed with a droplet reader which counts the number of fluorescence and empty droplets in a channel corresponding to the initial number of target molecule in the sample

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BMC Genomics

ISSN: 1471-2164

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