| End repair | Bead cleaning | A-tailing | Bead cleaning | Adaptor ligation | Bead cleaning | PCR & bead cleaning | Number of steps after shearing |
---|---|---|---|---|---|---|---|---|
NEBNext | x | x | x | x | x | x | x | 8 |
NEBNext Ultra | 2 in 1 a | Â | x | x | x | 5 | ||
SureSelect | x | x | x | x | x | x | x | 8 |
Truseq Nano | x | x | x | Â | x | x | x | 7 |
Truseq DNA PCR-free | x | xx b | x | Â | x | x | Â | 6 |
Accel-NGS 1S c | Adaptase | Â | 1st extension | x | 2nd extension | x | x | 7 |
Accel-NGS 2S c | 4 different steps + 4 bead cleaning | x | 10 | |||||
KAPA Hyperd | Â | 2 in 1 a | Â | Â | x | x | (x) | 3 (or 5) |
KAPA HyperPlusd,e | Â | Â | x | Â | x | x | (x) | 3 (or 5) |