Multiple adaptive routes of Salmonella enterica Typhimurium to biocide and antibiotic exposure

BMC Genomics

Table 4 Mutations identified in sequenced mutants

		TRI^RCHX^RBKC^R				BKC^R	TRI^HSCHX^RBKC^R	TRI^HS
Function	Gene	NE/TRI1	NE/CHX2	CHX/BKC3		TRI/BKC3	NE/BKC3	CHX/AMP
Amino acid/peptide transport/metabolism	asnA ^a						G → T/A74A
Energy production/amino acid metabolism	aarF ^b						T → A/L317Q
Lipid metabolism	fabI ^c	G → A/G93S
Membrane/transport	tolA ^d							T → A/V168E
Replication	ftsK ^e	T → G/P772P		A → T/Q780H	A → T/Q793H
Surface structures	mipA ^f						T → C/F61L
Surface structures	motB ^g					T → G/E128D
Virulence	bigA ^h		Deletion:408 bp			Deletion:331 bp		Deletion:293 bp
Intergenic	-/- ⁱ							T → A
	purH ^j /-		A → C				A → C
	tdcA/garA ^k					C → T
Unknown	yeaN ^l			C → G/T321T

Description of gene products: ^a Asparagine synthetase A, ^b Ubiquinone biosynthesis protein; ^c Enoyl-acyl carrier protein reductase (NADH); ^d Membrane transporter TolA protein; ^e Cell division protein FtsK; ^f Hypothetical outer membrane protein; ^g Motility protein B; ^h Hypothetical surface-exposed virulence protein; ⁱ Intergenic region between Glutamyl-t-RNA synthetase (459 bp)/(359 bp) Xanthosin operon transcriptional regulator; ^j Intergenic region between Phosphoribosylaminoimidezolecarboxamide fosmyltransferase and IMP cyclohydrolase/- (16S rRNA); ^k tdc operon transcriptional activator/Hypothetical surface-exposed virulence protein; ^l Hypothetical membrane protein of Major Facilitator Superfamily (transporter)

ISSN: 1471-2164