Fig. 4

RTqPCR validation of dysregulation in 13 genes identified by RNA-Seq analysis. Fold expression change relative to the mean expression level of the wild type mice, which is set at 1 in all cases. Error bars represent SEM (*p value ≤0.05, ** p value ≤0.002, *** p value ≤ 0.001, **** p value ≤ 0.0001). a Expression of Fgfbp1 and Arhgap8 was elevated in KO when normalized to the WT. No statistical difference was found in Aqp9. b Lrrn4 and Sp110 were elevated in KO when normalized to the WT. c Expression of Stat1, Fzd1, Tgfb2, Wnt11, Lhx2, Slc6a19, Myf6 and Krt2 was decreased in KO when normalized to the WT