EventPointer: an effective identification of alternative splicing events using junction arrays

BMC Genomics

Table 2 Top-ranked AS events regulated by SRSF1 and the result of their validation

Ranking	HGNC symbol	Type	Validation
1	MYCBP2	Complex Event	OK
2	KIF23	Cassette Exon	OK
3	AC024560.3	Cassette Exon	OK
4	FBXO22	Cassette Exon	OK
5	SRSF3	Complex Event	~
6	SUPT16H	Alternative Last Exon	OK
7	HMBOX1	Alternative First Exon	OK
8	ACAD11	Complex Event	OK
9	NCOR1	Cassette Exon	OK
10	AUP1	Retained Intron	OK
11	IFT27	Alternative Last Exon	OK
12	GALNT10	Alternative Last Exon	~
13	PARD3	Alternative Last Exon	~
14	PRMT2	Complex Event	OK
15	HORMAD1	Cassette Exon	OK
16	ANAPC7	Alternative Last Exon	OK
17	OGT	Complex Event	OK
23	MSL3	Alternative First Exon	OK
26	NT5C	Alternative 5′	OK
29	ALG2	Alternative First Exon	OK
33	MSL3	Alternative First Exon	OK
39	BAIAP2L1	Alternative First Exon	OK
40	HIST1H2AC	Alternative 5′	~
44	DDX52	Retained Intron	~
71	TMEM214	Retained Intron	~
74	GABPB1	Alternative 5′	OK
77	EIF3B	Retained Intron	~
101	LAMP1	Retained Intron	~
113	LMO7	Alternative 3′	OK
149	SCAMP3	Alternative 5′	X
226	UHRF2	Alternative 5′	X
355	COPS3	Alternative 3′	X
464	SLSC9A8	Alternative 3′	~
473	FLNA	Alternative 3′	X
480	C21orf58	Alternative 3′	OK
500	CALU	Mutually Exclusive	X
688	CCT6P1	Mutually Exclusive	~
844	ST20	Mutually Exclusive	OK
1388	ACO2	Mutually Exclusive	X
1956	KIAA0100	Mutually Exclusive	X

The first column shows the ranking of the event in EventPointer according to the p-value. The last column states if the validation was positive: a (OK) mark is shown if the validation is significant in PCR, a (~) mark is shown if differences in the PCR can be observed but the statistical significance is above 0.05. A (X) mark is shown if the event was not validated either because there was no differential splicing, no alternative splicing or no expression at all

ISSN: 1471-2164