Table 17 (abstract P84) The table depicts the results for the transformation efficiency, expression and toxicity studies of pRESTA-MAP1138c and pET28a-MAP1138c expression vector constructs in BL21DE3, pLysS and pLysE host cells
From: Abstracts from the 3rd International Genomic Medicine Conference (3rd IGMC 2015)
Expression vectors | Transformation efficiency (transformants/μg DNA) of strains | Toxicity Antibiotics + 1mM IPTG over night | Expression in liquid media (Antibiotics + 1mM IPTG for 3 h) | ||||||
|---|---|---|---|---|---|---|---|---|---|
BL21DE3 | BL21(DE3)-pLysS | BL21(DE3)-pLysE | BL21DE3 | BL21(DE3)-pLysS | BL21(DE3)-pLysE | BL21DE3 | BL21(DE3)-pLysS | BL21(DE3)-pLysE | |
pRSETA-MAP1138c | 1400 ± 100 | 14434 ± 208 | 16646 ± 150 | Yes | Yes | Yes | No | No | Yes |
Very Low | |||||||||
pET28a-MAP1138c | 3050 ± 50 | 24533 ± 416 | 2500 ± 208 | Yes | No | No | Yes | Yes | Yes |
Low | High | Low | |||||||