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Fig. 2 | BMC Genomics

Fig. 2

From: Forward genetic screen of human transposase genomic rearrangements

Fig. 2

Massively parallel DNA sequencing combined with PCR amplification for high-resolution mutational analysis of HPRT1. a Schematic of the human HPRT1 gene structure with vertical bars and horizontal arrows denoting exons and amplicons as numbered. b Photograph of ethidium bromide-stained and electrophoretically-resolved HPRT1 PCR amplicons of genomic DNA isolated from GFP (red) and GFP-PGBD5 (blue) expressing cells before (−) and after (+) thioguanine resistance selection

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