Table 2 Threshold cycle (CT): Values of average, standard deviation and coefficient of variation for tHKGs and nHKGs
From: A strategy to identify housekeeping genes suitable for analysis in breast cancer diseases
CT | GAPDH | B2M | ACTB | TUBA1A | 18S | CCSER2 | UBXN4 | SYMPK | TMEM11 | ANKRD17 |
|---|---|---|---|---|---|---|---|---|---|---|
MCF-7 | 18.51 | 23.97 | 16.24 | 21.92 | 20.49 | 29.72 | 26.61 | 29.46 | 27.04 | 28.82 |
MDA-MB-231 | 16.45 | 20.80 | 13.84 | 20.22 | 19.61 | 27.47 | 24.83 | 27.35 | 24.69 | 27.12 |
MDA-MB-468 | 20.48 | 25.49 | 18.09 | 24.94 | 23.47 | 30.96 | 30.49 | 30.84 | 29.44 | 31.57 |
T47D | 15.66 | 22.14 | 14.62 | 20.32 | 19.56 | 28.01 | 25.33 | 27.34 | 24.94 | 26.80 |
MCF10A | 18.29 | 23.32 | 17.56 | 22.69 | 17.86 | 29.96 | 28.61 | 29.74 | 27.98 | 29.25 |
Minimum | 15.67 | 20.81 | 13.85 | 20.23 | 17.87 | 27.47 | 24.84 | 27.35 | 24.69 | 26.81 |
Maximum | 20.49 | 25.50 | 18.10 | 24.94 | 23.47 | 30.96 | 30.50 | 30.84 | 29.45 | 31.57 |
Mean | 17.88 | 23.15 | 16.07 | 22.02 | 20.20 | 29.23 | 27.18 | 28.95 | 26.82 | 28.72 |
Std. Deviation | 1.892 | 1.782 | 1.831 | 1.942 | 2.062 | 1.444 | 2.359 | 1.550 | 2.021 | 1.912 |
Coefficient of variation (%) | 10.58 | 7.70 | 11.39 | 8.82 | 10.21 | 4.94 | 8.68 | 5.35 | 7.53 | 6.66 |