Fig. 4

The number of DE genes used in characterization of mouse immune response against S. mansoni infection (High dose infection: H, low dose infection: L and uninfected: U; 2 mice per group). Four separate master transcriptomes were assembled using de novo (Trinity de novo and SOAPdenovo–Trans) and reference based (Genome guided Trinity and Cufflinks) approaches. Thereafter the non-target transcripts were identified and removed. Differential expression was calculated using DESeq2, EdgeR, and EBSeq and for each assembly shared predictions were extracted. The figure represents the resulting differentially expressed genes corresponding to the four assembly approaches, after removing the duplicates between assemblies